细胞代谢调控在肿瘤的发生发展中至关重要，其相关研究已成为当今肿瘤研究的热点。我们的前期研究显示：代谢性核受体PPARα可能是调控胶质瘤细胞糖代谢和生长的重要抑癌因子，其与转录因子E2F1、微小RNA miR-19a三者具有相关性，三者间可能构成反馈环路调控胶质瘤细胞代谢和生长。本课题拟基于临床大样本胶质瘤基因组学数据库平台构建和数据整合分析，结合体外细胞功能验证、分子水平miRNA靶基因验证、转录调控验证、回复实验及体内动物模型，解析E2F1/miR-19a/PPARα反馈环路在胶质瘤细胞代谢和生长过程中的调控作用及其机理，并构建PPARα靶分子调控网络，探索胶质瘤基因调控网络中的多层级分子共调控网络轮廓，为基于E2F1/miR-19a/PPARα反馈环路的胶质瘤诊断和治疗提供依据。

Regulation of cell metabolism influences all biological processes in organisms and plays a key role in tumor progression. Our previous study demonstrated that Peroxisome Proliferator-Activated Receptor (PPAR) α may influence cell glucose metabolism and growth as a suppressor factor in human glioma as well as constitutes a feedback loop with E2F1, a transcription factor and miR-19. Thus, we proposed the hypothesis that PPARαregulation network based on E2F1/miR-19a/PPARαfeedback loop plays a key role in glioma glycine metabolism and growth. In the future study, we will use transcriptional regulation, posttranscriptional regulation, gene rescue, data integration analysis and animal models as major methods to identify the E2F1/miR-19a/PPARαfeedback loop, explore the PPARα regulation network in glioma, and build the multi-level molecular in glioma gene network. In conclusion, our finding will reveal the role and mechanism of E2F1/miR-19a/PPARαfeedback loop in glioma glycine metabolism and growth, and provides more potential approaches for glioma diagnosis and treatment.