胶质瘤是中枢神经系统肿瘤中最常见而又最难治疗的恶性肿瘤。最近的研究表明，胶质瘤干细胞的存在和胶质母细胞瘤的放疗和化疗抵抗相关，导致患者预后不良。针对胶质瘤干细胞的靶向给药系统治疗有望从根本上提高胶质瘤的治疗效率。本研究拟以胶质瘤干细胞为靶标通过体外筛选技术获得高亲和力和高特异性识别胶质瘤干细胞的核酸适体学列；结合热力学计算手段和核酸合成方法，对所获序列进行测序和结构优化，获得最优优序列；通过蛋白质质谱技术发现和表征胶质瘤干细胞表面特征生物标记物；并且建立以核酸适体为载体以胶质瘤干细胞为靶点的新型胶质瘤靶向药物治疗方法。本项目从化学和化学生物学的新角度出发，获得胶质瘤干细胞特异性识别分子和胶质瘤干细胞生物标志物，开发胶质瘤干细胞靶向给药体系，为揭示胶质瘤的发生与发展机制及靶向治疗和创新性药物的开发提供理论依据与物质基础.

Glioma is the most common and most lethal form of brain tumor in human. Recent data indicate that glioma stem cells are responsible for resistance of glioblastomas to radiotherapy and chemotherapy, thereby contributing to the poor survival of these patients.The development of a targeted therapy system specifically against glioma stem cells would significantly improve treatment efficiency. Using glioma stem cell as target, this project will apply in vitro selection techique to discover aptamer sequences that bind to glioma stem cells with high affinity and high specificity;optimal atpamers sequences will then be obtained via the combination of binding thermodynamic simulation and the nucleic acid synthesis method；using protein mass spectroscopy glioma stem biomarker on cell surface will be discovered and characterized； finally the aptamer will be used as a carrier for targeted drug delivery against glioma. The study from a new angle of chemical biology will establish a new method for indentifying biomarker for glioma stem cells, provide theory evidence and material foundation of elaborating the mechanisms of development of glioma and targeted therapies and innovative drug development.