近红外CuInSe2量子点的制备及其在肿瘤成像上的应用研究

针对目前肿瘤在体荧光成像诊断技术存在背景荧光信号而造成的灵敏度低，所用探针缺乏特异性而造成的靶向性差，荧光探针中的荧团发射波长处于可见光区而造成的深部组织显像不灵敏等缺陷。本课题拟在前期工作基础上，以溶胶制备低毒CuInSe2纳米晶技术为支撑，以CuInSe2的缺陷调控为手段，通过适当的掺杂及对表面进行功能化修饰改性，制备得到高稳定性、高生物相容性、高量子产率的CuInSe2近红外量子点。通过生物偶联技术与肿瘤靶向性配体偶联构建具有生物靶标活性的近红外量子点荧光探针，应用于肿瘤细胞成像和活体靶标成像方面，从而推动荧光成像技术在肿瘤诊断、治疗和监测中的发展。

To date, in vivo tumor fluorescence imaging, including low signal-to-noise caused by the background fluorescence, poor targeting capability caused by the lacking of specific ligands, insensitive deep tissue imaging caused by the fluorophore emission wavelength in the visible area. Based on the previous researches, we will synthesize the high quantum yield CuInSe2 near-infrared quantum dots (NIR QDs) in this project, which supported by sol preparation low-toxic CuInSe2 nanocrystals technology and by means of the defects of CuInSe2 regulation, following with doping and surface functionalization of the as-prepared CuInSe2 QDs to achieve high stability and good biocompatibility. Fabrication of near-infrared fluorescent quantum dots-based biological activity probes through biological coupling technique and tumor targeting ligands coupling, which applied to the tumor cells and the targets in vivo imaging, and to promote the development of fluorescence imaging in the diagnosis, treatment and monitoring of tumor.