LncRNA-AK025743.1-miR-194环路在大肠癌发生发展中的作用和机制研究

大肠癌的发生率和死亡率居于前列。现已知长链非编码（long non coding，lnc）RNA在多种肿瘤中发挥重要作用，但在大肠癌发生发展中的作用及其机制未明。我们已发现lncRNA-AK025743.1在大肠癌组织中高表达，敲低AK025743.1后可抑制大肠癌细胞增殖及侵袭，上调miR-194并抑制其下游基因Akt 2的表达，而过表达miR-194可下调AK025743.1。因此我们推测AK025743.1与miR-194相互作用，调控PI3K/AKT信号通路从而促进大肠癌发生发展。本研究将从细胞功能、临床标本、动物实验等方面探讨LncRNA-AK025743.1-miR-194环路影响大肠癌发生发展的具体机制；明确AK025743.1作为新靶点诊治大肠癌的意义。

Colorectal cancer is one of the most common neoplasm disease with a high mortality and incidence rate. Long non coding (lnc) RNAs have been known to play an important role in several malignant diseases; however, the mechanism of lncRNAs in colorectal cancer tumorigenesis is still unknown. LncRNA-AK025743.1 was found to be overexpressed in human colorectal cancer in our previous studies. Downregulation of AK025743.1 significantly inhibited cell proliferation and invasive ability, meanwhile increased the expression of miR-194 and restrained its downstream target gene Akt2. However, overexpression of miR-194 downregulated AK025743.1. Therefore, we assume that AK025743.1 interacts with miR-194 and promotes colorectal cancer tumorigenesis through PI3K/AKT pathway. We will explore the mechanism of LncRNA-AK025743.1-miR-194 loop in colorectal colorectal cancer tumorigenesis via in vitro, in vivo experiments and patient samples; determine the significance of AK025743.1 as new target in clinical practice.