基于衰老标记蛋白30和自噬交互介导氧化应激探讨七宝美髯丹对小鼠皮肤光老化的作用机制

皮肤衰老影响美观，是医学美容关注的热点。研究发现氧化应激伴增龄而增强，促使皮肤细胞活性氧堆积，是导致内外源性皮肤衰老的共同机制。中医认为肝肾亏虚、精血耗伤是皮肤衰老的重要原因，滋补肝肾、填精养血是延缓皮肤衰老的有效治法。古方七宝美髯丹，临床应用多年，具有滋补肝肾、填精养血之功效，是体现该治法的方药，并能抑制氧化应激，促进皮肤年轻化，但作用机制有待深入。本项目基于已结题青年基金成果（SMP30与细胞自噬交互作用共同抑制氧化应激损伤，延缓细胞衰老），建立小鼠皮肤光老化模型，同时七宝美髯丹干预，并培养模型鼠原代皮肤成纤维细胞，借助基因沉默、基因敲除等手段，通过免疫荧光、分子生物学、透射电镜等技术检测基于SMP30与细胞自噬交互介导氧化应激，调控衰老、自噬等相关指标，旨在研究七宝美髯丹对皮肤光老化的作用机制，为七宝美髯丹的临床应用提供科学依据。

Skin aging is the focus of medical cosmetology.Research found out that oxidative stress injury which enhanced with aging increased the express of reactive oxygen species in skin cells, as a result, causing skin aging. Due to the theory of Chinese medicine, deficiency of liver-kidney and essence is an important incentive for the formation of skin aging. While in clinical practice, liver-kidney nourishing and essence strengthening is one effective treatment of delaying skin aging. A Chinese medicine named QI Bao Mei Ran Dan is a classic Chinese prescription for such application. With the function of nourishing liver-kidney and strengthen essence, it can effectively treat the skin aging, how ever, pharmacology and mechanism of this medicine had yet to be further developed. Based on the pervious research by us, we would build three mouse photoaging models: Ultraviolet, Ultraviolet with SMP30 silencing, Ultraviolet with SMP30 knockdown. At the same time, we pretreat the models by QI Bao Mei Ran Dan. To acquire cultured rat skin fibroblasts. We detect the mark of autophagy and aging during the SMP30 mediated skin aging in primary fibroblast which getting from the mouse models. These researches should explore the target and molecular mechanism of QI Bao Mei Ran Dan in reducing the photoaging skin。