基于miR-155-SOCS1-JAK2/STAT3的双抑制信号通路与Th17/Treg失衡的相关性探讨蜈蚣败毒饮治疗银屑病的机制研究

银屑病病机复杂，申请人在以往血分辨治的基础之上，从病由毒生之“毒”和久病必瘀之“瘀”入手，重新阐明银屑病“毒蕴瘀结”核心病机及“两极阻断学说”。以“解毒消源，祛瘀截末”治则创立融入未病防变思想的“蜈蚣败毒饮”。在既往因子、细胞、基因、蛋白质组学及Th1/Th2漂移到Th17细胞探索的同时，申请人发现，既往研究的Th17细胞参与细胞失衡，STAT3是JAK2/STAT3的重要组分，中药药理学亦与之相关，因此提出“蜈蚣败毒饮可能通过干预miR-155-SOCS1-JAK2/STAT3的双抑制信号通路而调控Th17/Treg细胞失衡而治疗银屑病”的假说，拟分别探讨抑制源头miR-155，次级抑制因子SOCS1，通路核心JAK2/STAT3，Th17/Treg轴及差异性蛋白，针对“一通路，两抑制，两细胞，多蛋白”的研究框架，整合通路与T细胞亚群的交叉关联，完善蜈蚣败毒饮治疗银屑病的T细胞网络机制。

The pathogenesis of psoriasis is complex, the applicant starts with the “Poison” that causing disease and the “Stasis” caused by chronic disease, and re-clarifies the core pathogenesis which is “Poison Containment and Stasis Accumulation” and puts forward the “Two Poles Blocking Theory” on the basis of previous studies of psoriasis from the “Blood”. Founding the prescription of “Wugong Baidu Decoction” that integrating into the thought of “Preventive Changes without Disease” based on the principles of “Detoxication to Eliminate the Source, Removing Stasis to Cut the End”. On the basis of previous researches which including factors, cells, genes, proteomics and Th1/Th2 drift to Th17, applicant found that Th17 cell previously studied is involved in cells unbalance, STAT3 is an important component of JAK2/STAT3 and pharmacology of drug components is also associated with it, therefore, research group put forward the hypothesis that “Wugong Baidu Decoction may be used to treat psoriasis by regulating the imbalance of Th17/Treg cells through intervention double inhibitory signal pathway of miR-155-SOCS1-JAK2/STAT3”, and to be discussed separately the miR-155 of inhibition source, SOCS1 of secondary inhibitor, JAK2/STAT3 of access core, Th17/Treg axis and differential proteins, aiming at research framework of “One Pathway, Two Inhibitors, Two Cells, Multi Proteins”, integrating cross-linkages between pathway and T cell subsets, improving T cell network mechanism of Wugong Baidu Decoction in the treatment of psoriasis.