基于冷应激和动态饲喂模式研究羔羊细胞自噬调控肌纤维类型转化的分子机制

寒冷是北方高寒地区动物面临的一种最普遍的应激因素，使得断奶羔羊处于冷应激状态，造成饲料转化率低，严重影响肌纤维的发育及肌纤维类型的转化，降低产肉能力和肉品质，给草原畜牧业造成重大经济损失。本项目采用体内和体外结合的方法，基于冷应激和动态饲喂模式条件下，选取羔羊肌肉为对象，研究肌细胞自噬调控肌纤维类型转化的分子机制。拟寻求冷应激条件下降低肌肉氧化应激损伤的最佳饲喂模式，揭示冷应激和动态饲喂模式对羔羊肌纤维类型转化的影响，阐明冷应激和动态饲喂模式下羔羊肌细胞自噬调控肌纤维类型转化的分子机制，以期为提高羊肉产量及改善肉品质提供一定的理论依据。

Cold is a common cold-stress factors in the regions of the North, which led to reduce the feed digestibility, meat production and quality, and affect the muscle fiber development and change of styles for weaning lambs in a state of cold-stress. It will be an significant economic losses to the grassland animal husbandry. Based cold-stressed and dynamic diets, we select muscle as a model and analysis the molecular mechanisems on cell autophagy regulating the change of muscle fiber styles in weaning lambs in vivo and vitro methods. The purpose is to try to find the better feeding mode to reduce oxidative stress damage in muscle under cold-stress. The results may explain that the effects on the change of muscle fiber styles, and molecular mechanisems on cell autophagy regulating the change muscle fiber styles in weaning lambs by cold-stressed and dynamic diets. It is helpful to provide the theoretical basis for improving mutton production and quality.