我们研究发现HOXB9在肝细胞癌的侵袭和转移过程中发挥重要作用，但HOXB9被调控机制尚未完全阐明。前期研究发现eEF1A1在恶性肿瘤的进展中起重要作用《Cancer research，2016》。预实验发现：eEF1A1在肝癌组织中过表达，且与HOXB9表达呈正相关；干扰肝癌细胞中eEF1A1表达可下调HOXB9表达，同时肿瘤细胞侵袭迁移能力下降。因此，我们推测eEF1A1通过调控HOXB9表达影响肝癌的侵袭转移。为验证这一假设并探讨其作用机制，本研究拟先采用RNA干扰等技术明确eEF1A1通过调控HOXB9表达对肝癌体外侵袭及体内转移的影响；再利用荧光素酶报告基因及染色质共沉淀等方法探讨eEF1A1调控HOXB9的具体机制；最后通过检测肝癌组织中eEF1A1和HOXB9表达，分析两者表达的关联及作为肝癌术后转移和预后标志物的意义。本研究结果将为阐明肝癌的侵袭和转移机制提供新的理论依据。

Our study found that HOXB9 plays an important role in the invasion and metastasis of hepatocellular carcinoma (HCC), but the regulatory mechanism of HOXB9 has not been fully elucidated. Previous studies have found that eEF1A1 plays an important role in the progression of malignant tumors (Cancer research，2016). In our previous study, some phenomenon were found: eEF1A1 was overexpressed in HCC tissues and positively correlated with HOXB9 expression; Knockdown of eEF1A1 expression can down-regulate HOXB9 expression and decrease the invasive and migratory ability of HCC cells. Therefore, we speculate that eEF1A1 affects the invasion and metastasis of HCC by regulating HOXB9 expression. To confirm this hypothesis and explore its mechanism, RNA interference and other techniques are used to clarify the effect of eEF1A1 on the invasion and metastasis of HCC in vitro and in vivo by regulating HOXB9 expression. Then, luciferase reporter gene assay and chromatin immunoprecipitation (ChIP) are used to explore the specific mechanism of eEF1A1 regulating HOXB9. Finally, by detecting the expression of eEF1A1 and HOXB9 in HCC tissues, the correlation between the expression of eEF1A1 and HOXB9 and their significance as markers of post-operative metastasis and prognosis of HCC are analyzed. The results of this study will provide a new theoretical basis for elucidating the mechanism of invasion and metastasis in HCC.