黔产3种凤尾蕨属植物中抗HIV活性二萜的研究

AIDS是威胁人类生命健康的重大疾病之一，也是全球性攻克难题。迄今为止，尽管还没有较好的治疗方法和药效肯定的药物来抗衡AIDS，但世界各国的医药科学工作者为此仍在坚持不懈的努力，坚信能有战胜AIDS的时刻。申请者与研究生从狭叶凤尾蕨中分离到新化合物Henrin A，进行活性筛选时发现：Henrin A 具有抗HIV/AIDS的活性（论文发表在Int. J. Mol. Sci.期刊上）。实验结果给予了我们两点提示：1、凤尾蕨属植物中Henrin A 类型的小分子衍生物是否具有更好的抗HIV/AIDS的活性？2、该属植物是否还有其他类抗HIV/AIDS的活性成分？我们拟采用LC-MS在线检测和pre-HPLC等方法从凤尾蕨属植物中快速发现和分离二萜类化合物，并通过体外实验对化合物进行抗PR、RT、IN活性筛选，解决上述提出的两个科学问题，为寻找和发现抗艾滋病药物拓宽思路和研究方法。

AIDS is one of the major diseases threatening human life and health, is also a global conquer difficulties. So far, although there is no better treatment methods and efficacy of certain drugs against AIDS, but the world of medical scientific workers are still persistent their efforts, firmly believe that they can cure AIDS. One new compound was isolated from Pteris henryi, named Henrin A, which showed anti-HIV activity. The experimental results gave us two hints. Whether there are better activity derivatives of Henrin A in the Pteris. Whether there are other anti-HIV/AIDS compounds in the plants. We will find and isolate diterpenoids from the Pteris plants by LC-MS online screening and pre-HPLC. The isolated compounds will be evaluated for their anti-PR, anti-RT, anti-IN activities in vitro. This topic will broaden the train of thought and research methods for seeking and finding more anti-HIV drugs.