骨髓间充质干细胞（BMSCs）移植治疗股骨头坏死越来越受到关注。研究发现，在股骨头坏死修复过程中，低氧诱导因子-1α（HIF-1α）和基质细胞衍生因子-1α（SDF-1α）发挥重要的作用。本项目拟采用HIF-1α转染的BMSCs为种子细胞，联合负载SDF-1α的表面粗糙可降解PLGA微球作为移植基质治疗兔激素性股骨头坏死模型。转染HIF-1α的BMSCs能够分泌更多的VEGF，并介导更多的BMSCs迁移到骨坏死区；负载SDF-1α的表面粗糙可降解微球，能够促进BMSCs更快铺展和增殖，并且微球降解时缓慢持续释放的SDF-1α可以诱导更多外周血中的干细胞归巢到骨坏死部位，抑制内皮细胞和骨细胞凋亡，促进血管生成和骨形成。本项目通过ELISA、RT-PCR及Western-blot等方法及X片及MRI等影像学检测，探讨上述方法治疗激素性股骨头坏死的疗效，为股骨头坏死非手术治疗提供实验依据。

More attention pay for mesenchymal stem cells'(BMSCs) transplantation treating for avascular femoral head necrosis(ANFH). The study found that hypoxia-inducible factor-1a(HIF-1α) and stromal all derived factor-1α(SDF-1α) play an important role in the repair process of ANFH. This project intends to focus on BMSCs modified by HIF-1α as seed cells, and combine with roughly biodegradable PLGA microspheres loaded SDF-1α as the substrate traeting for the early ANFH. BMSCs transfected HIF-1α can secrete more VEGF, and mediate more BMSCs migrating to the site of bone necrosis. The roughly biodegradable PLGA microspheres loaded SDF-1a can promote BMSCs spreading and proliferation faster, and the microspheres slowly sustained release can home more peripheral blood stem cell to the site of bone necrosis, and inhibit the apoptosis of endothelial cells and bone cells, also promote angiogensis and bone formation. This project using ELISA, RT-PCR, Western-blot, X-ray and MRI methods, investigate the efficacy of above method treating for early osteonecrosis, and provide the basis for the non-surgical treatment for femoral head necrosis.