多氯联苯微生物降解的土壤胶体界面作用机制研究

多氯联苯（PCBs）是国际上共同关注的优控持久性有机污染物。土壤中复合胶体是影响PCBs微生物降解的重要限制因子，故土壤胶体-PCBs-降解微生物之间的相互关系成为探讨PCBs污染土壤微生物修复机理的关键内容。本项目以不同类型土壤提取的复合胶体与PCBs降解根瘤菌为实验材料，采用13C-PCBs稳定同位素与gfp基因双标记示踪法，借助等温滴定微量热(ITC)、傅立叶变换红外光谱（FTIR）、核磁共振（NMR）、扫描电子显微镜（SEM）、激光共聚焦显微镜（LSCM）、原子力显微镜（AFM）等现代仪器分析手段，深入研究根瘤菌与土壤复合胶体的相互作用，探讨根瘤菌存在对PCBs与土壤胶体相互作用的影响，阐明PCBs与微生物在土壤复合胶体界面上的相互作用机制，为多氯联苯污染土壤的微生物修复提供科学依据。

Polychlorinated biphenyls (PCBs), as one of the priority persistent organic pollutants (POPs) , are of great concern due to their persistence and toxicity in the world. Soil complex colloids are the most important limiting factor influencing the microbial degradation of PCBs, and thus the interrelation among soil colloids, PCBs and degrading microbes has become the key content to investigate the microbial remediation mechanisms of soil contaminated by PCBs. This project will be carried out based on the experimental materials of soil colloids prepared manually and extracted from natural soils and Rhizobium meliloti which has been proved to be degrading bacteria of PCBs. By 13C-PCBs isotope, gfp gene double mark, and modern instrumental analysis methods such as isothermal titration microcalorimetry(ITC), Fourier transform infrared spectrum (FTIR), Nuclear magnetic resonance (NMR), Scanning electron microscope (SEM), Laser scanning confocal microscope (LSCM), Atomic force microscope(AFM) and so on, the interaction between Rhizobium meliloti and soil colloids will be deeply investigated, the effect of Rhizobium meliloti on the interaction between PCBs and soil colloids will be discussed, and the interaction mechanism between PCBs and Rhizobium meliloti on the interface of soil colloids will be revealed, which will provide the scientific basis for bioremediation of soils contaminated with PCBs.