脊髓损伤发病率高，给患者及社会造成了沉重的负担，目前尚无公认的有效治愈办法，中医电针治疗脊髓损伤具有较好的疗效，但其作用机制尚不明了。既往研究表明，电针督脉大椎、命门两穴能调控脊髓P53的表达，P53的直接靶点miRNA-34a能调控神经干细胞分化，电针刺激p53/miRNA-34a轴调控脊髓神经干细胞分化可能是电针治疗脊髓损伤的重要机制。本研究通过体内、外实验，采用行为学评价，分子生物学以及细胞生物学等研究方法，研究电针刺激p53/miRNA-34a轴影响细胞增殖、凋亡和炎症反应等，改善脊髓微环境，直接或间接调控神经干细胞分化以治疗脊髓损伤，促进脊髓功能恢复的作用机制。

The high incidence of spinal cord injury(SCI) to patients and the community caused a heavy burden, there is no recognized effective cure, electroacupuncture treatment of spinal cord injury has a good effect, but its mechanism of action is not yet clear. Previous studies have shown that electroacupuncture at Dazhui and Mingmen two acupoints can regulate the expression of P53 in the spinal cord. MiRNA-34a,the direct target of P53, can regulate the differentiation of neural stem cells, and the acupuncture stimulation of p53 / miRNA-34a axis may regulate the differentiation of neural stem cells, this may be the important electroacupuncture mechanism for SCI. In this study, we investigated the mechanism study of stimulation effect of electroacupuncture on neural stem cell dedifferentiation through p53/miRNA-34a axis for the treatment of SCI by vivo and vitro experiments, use methods including evaluations for cell proliferation, apoptosis and inflammatory response,changes of spinal cord microenvironment, as well as behavioral evaluation.