胡麻wrinkled1基因的克隆及功能分析

在胡麻（Linum usitatissmu）的遗传育种方面，提高其含油量是最主要的问题之一。在种子脂肪酸代谢的调控中，与仅对单个步骤进行遗传修饰相比，通过转录因子对参与目标代谢途径的一组基因进行遗传修饰，可以更有效地获得目标表型。近年来，对转录因子WRINKLED1研究较多，在其它油料作物中的研究表明，WRI1在种子油的积累过程中起着关键性的作用，它可能是种子脂肪酸代谢途径中重要的调控因子。因此，本研究拟克隆胡麻的LuWRI1基因，用DNA重组技术构建LuWRI1过表达载体和RNAi干涉载体。用LuWRI1过表达载体转化拟南芥缺失突变体wrinkled1；用过表达载体和RNAi干涉载体转化胡麻。通过对转基因后代种子的表型观察、含油量测定以及脂肪酸组份的分析，阐释胡麻LuWRI1基因的功能，为进一步通过分子育种开展胡麻高油种质的创新奠定研究基础。

To increase oil content is one of the most important issues in Flax (Linum usitatissmu) breeding. In the regulation of metabolism of fatty acids in seeds, comparing with a single modification in one step, it's more effective to get target phenotypes through transcriptional factor to modify genetically a group of genes involved in the targeted metabolic pathways.In recent years, rsearch on the transcriptional factor of WRINKLED1 shows that WRI1 plays a crucial role in the process of oil accumulation in other oilseeds except flax.WRI1 may be an important regulator in fatty acid metabolic pathway of seed. Therefore, this study will clone the gene of LuWRI1 from flax, and construct LuWRI1-overexpressed vector and RNAi interference vectors using recombinant DNA technology.Then the overexpressed vector will be transformed into a mutant of Arabidopsis thaliana with deleted wrinkled1. The overexpressed vector and RNAi interference vectors will also be transformed into flax. Lastly, phenotype, oil content, and fatty acid of transgenetic offspring will be analyzed in order to explain the function of LuWRI1, and futher provide research basis for molecular breeding to creat a new flax with high oil content.