基于血小板微颗粒信使作用研究桃红四物汤对实验性脑缺血血管新生调节机制

缺血区脑微血管新生是祛瘀生新中药调控脑缺血损伤的重要环节，血小板在脑缺血状态下可以释放携带特异生物因子的血小板微颗粒（PMPs），并以PMPs为信使调节微血管内皮细胞功能，影响脑微血管新生。祛瘀生新经典复方桃红四物汤临床对于缺血性脑中风有明确的作用，前期研究发现其有调节脑缺血大鼠血小板功能和促进血管新生作用。提示PMPs可能为桃红四物汤促进血管形成的作用靶点。本项目旨在探讨桃红四物汤对脑缺血状态PMPs携带生物信息特异性调节和特异性PMPs对脑微血管新生作用机制。通过建立脑缺血模型，考察桃红四物汤对脑缺血损伤保护和血管新生作用，同时提取血浆中血小板微颗粒并考察其数量和携带生物信息分子特征；通过建立脑微血管内皮细胞体外模型，考察桃红四物汤干预的脑缺血PMPs对脑微血管内皮细胞增殖和血管生长因子表达以及传导信号通路影响，明确桃红四物汤通过调节PMPs信使功能促进血管新生的调节机制。

Microvascular angiogenesis in ischemia area is an essential link of regulating cerebral ischemia by traditional Chinese medicine which have a therapeutic principle of “Eliminating blood stasis and promoting tissue regeneration”. When under the state of cerebral ischemia, platelets could shed special biological platelets microparticles(PMPs) that contain biological signal factors and , and regulate the function of brain microvascular endothelial cells(BMEC) as messengers . The therapeutic effect of Taohong-siwu decoction (THSW) has been proved in the aspect of treating cerebral ischemia.Our previously research found that THSW could regulate function of platelet and promot angiogenesis. So we want to explore weather THSW can promot angiogenesis in ischemic area via regulate the signal factors carried by PMPs. The aim of this study is to investigate the special regulation by THSW to the biological information carried by PMPs in cerebral ischemia, and study the mechanism of the microvascular angiogenesis by sepcial PMPs. First, rats cerebral ischemia model of rats was established, on which the angiogenesis effects of THSW were observed and the platelet microparticles were extract from plasma to analysis quantity and the transfer information. Second, detect multiplication and vascular endothelial growth factor induction by PMPs in brain microvascular endothelial cells and its downstream transduction pathway, in order to illuminate the mechanism of angiogenesis by adjusting the messenger function of PMPs.