基于聚乙二醇预聚物直接光刻制备抗蛋白吸附芯片电泳的研究

作为微全分析系统的芯片电泳在生命科学、生物医学工程和食品检验等领域，尤其是在蛋白质、DNA等生物分子的分离分析和微量制备等方面都显示出巨大的潜力和极为重要的应用前景。日新月异的应用要求对芯片电泳的制备和性能提出了新的挑战，如何能够简化芯片电泳和表面修饰功能涂层的制备过程，快捷构筑高质量、抗蛋白吸附性能优异的芯片电泳成为一个备受关注的问题。本课题旨在探讨如何利用聚乙二醇预聚物的直接光刻取代传统聚合物材料芯片电泳的模板制备，通过对抗蛋白吸附聚合物芯片电泳材料和微加工方法的创新，建立具有结构可控、适合多种蛋白质分离的芯片电泳直接光刻构筑的系统方法，利用聚乙二醇预聚物独特的光固化交联反应、光刻和抗蛋白吸附特性实现高性能聚合物芯片电泳的快捷微加工，并研究所制备的聚乙二醇芯片电泳的物理化学性质，探索其在蛋白质、多肽电泳分离和分析等方面的应用。

As a micro total analysis system, microchip electrophoresis (ME), featured in small, fast analysis, low cost, high integration density and automatic level, easy to carry and commercialization, have attracted considerable attentions from all over the world. ME has been widely used in the areas of environmental protection, biochemistry, medicine and health, clinical testing, judicial expertise, food sanitation, pharmaceutical checking, drug testing, agrochemistry, biomedical engineering and life science. As the applications of ME developed, how to facilely fabricate ME with excellent anti-protein-fouling properties is becoming one of the hottest areas. In this proposal, we will establish a systematic method to fabricate ME by direct photolithography of polyethylene glycol (PEG) prepolymers without using any templates or molds. The unique photo-crosslinking and anti-protein-fouling properties of the PEG prepolymers will be used to fabricate high performance ME facilely. The preparation mechanisms and properties of the PEG based anti-protein-fouling ME will be studied, and the applications in protein and polypeptide separation will be investigated.