偏穗鹅观草着丝粒序列分析及与小麦CENH3功能整合分析

着丝粒是真核生物染色体的重要组成部分，在多倍体进化中为不同基因组统一协调的重点区域之一，对它的研究有助于理解和认识多倍体的形成和进化研究，为远缘杂交奠定理论基础。小麦族是麦类作物远缘杂交育种的基因库，已成为改良麦类作物高产、抗病和抗逆等性状的重要基因资源。.本研究以小麦族偏穗鹅观草（Roegneria komarovii (Nevski) Nevski ，2n=4X=28，StStYY）为主要对象，构建BAC文库，为StY基因组其它研究搭建平台，筛选StY基因组着丝粒相关BAC克隆，构建亚克隆文库并测序分析，寻找StY基因组着丝粒区域功能性核心序列元件，探讨鹅观草属中StY多倍体物种的形成及其基因组分化，比较小麦和偏穗鹅观草的着丝粒结构异同，为小麦远缘杂交育种提供理论依据，提高远缘杂交育种的效率。

Centromeres maintain the integrity of chromosomes during cell division, Therefore, the DNA composition at the centromeric and pericentromeric regions can potentially provide valuable information for the elucidation of the evolution and speciation during the formation of polyploids..The Triticeae Damort. family are the most successful relative species in wheat distant hybridization breeding as an important genetic resource for improving the high yield, disease resistance and stress resistance of wheat crops. In this study, Roegneria komarovii (Nevski) Nevski, (2n = 4X = 28, StStYY) is the main target, we will Construct a BAC library of StY genome as research platform, screening centromere-associated BAC clones, construct sub-clone library to sequencing, find the functional sequence elements of the StY genome in centromere region, reveal the forming and evolution of polyploidy, understanding the centromere structure between wheat and Roegneria komarovii provide the theoretical basis of distant hybrid breeding in wheat.