小鼠成熟精子中tRNA来源的小非编码RNA（tsRNAs）已被证实具有传递表观遗传信息的重要功能。我们前期工作发现小鼠成熟精子中一个之前被忽视的，来源于28S rRNA的新小非编码RNA（rsRNA-28S），其丰度高于tsRNAs，并会响应生殖道炎症环境而改变表达。据此，我们希望深入探索rsRNA-28S在小鼠精子细胞中的表达特性和生物学功能。我们将探索和完善对生理情况下小鼠各发育阶段精子细胞rsRNA-28S在表达量、细胞定位和RNA修饰方面的认识，以及附睾炎病理条件下其所发生的改变。我们将进一步研究精子rsRNA-28S在受精卵和子代发育过程中的生物学功能，以及附睾炎精子rsRNA-28S的改变对其发育的影响。本研究将拓展精子中sncRNAs种类和功能的认识，并有助于更好地认识男性精子发生和成熟过程的生理病理机制，并为男性不育的诊断和治疗提供参考。

The tRNA-derived small RNAs (tsRNAs) in the mature sperm of mice have been shown to play an important role in the epigenetic inheritance. Our previous study discovered a previously overlooked 28S rRNA-derived small non-coding RNA (rsRNA-28S) in the mature sperm of mice, with a higher abundance than tsRNAs, and changes in expression in response to genital tract inflammations. .Based on these lines of evidence, we seek to further explore the expression patterns and biological functions of the rsRNA-28S in the mouse sperm cells. We plan to 1. characterize its expression levels, cell localizations and RNA modifications in the different developmental stages of the sperm cells, and their changes in the pathological conditions of epididymitis; 2. explore its biological functions in the development of zygotes and offsprings, and how the changes in sperm rsRNA-28S from the epididymitis model will affect the process. We hope to widen the knowledge regarding the types and functions of sperm sncRNAs, and meanwhile, to provide new biological insights to the physiological and pathological mechanisms underlying male germ cell development as well as the diagnosis and treatment of male infertility in this project.