玻连蛋白（Vitronectin)是细胞外基质的重要组成成分，已被广泛应用于人诱导多能干细胞（hiPSC）的维持和扩增，但其在造血发育中的作用却鲜有报道。我们前期研究发现，与小鼠基质胶Matrigel相比，Vitronectin能显著促进hiPSC早期造血分化。通过化学成分明确的Vitronectin高效地诱导hiPSC定向造血分化，为血细胞治疗带来了新的策略。本项目拟研究：1）细胞水平上，明确Vitronectin对hiPSC来源的中胚层祖细胞，生血内皮细胞及造血祖细胞分化的影响；2）分子水平上，以整合素αvβ3为切入点，深入探索Vitronectin调节hiPSC定向造血分化的分子机制；3）功能水平上，研究Vitronectin及其介导的αvβ3对造血祖细胞体内外功能的影响。本课题将为人正常造血发育研究及血细胞治疗研究奠定方法学和理论学基础，具有潜在的临床应用前景。

Vitronectin, an important component of the extracellular matrix，has been widely used for the maintenance and expansion of human induced pluripotent stem cells (hiPSCs). However, the effect of vitronectin on hematopoietic development remains unclear. Our previous studies showed that vitronectin significantly promoted early hematopoiesis of hiPSCs. Generation of blood cells from hiPSCs by chemically-defined vitronectin induction would offer a new strategy for blood cell therapy. So we intend to carry out our study as below : 1) At the cellular level, determining the effect of vitronectin on the development of mesodermal progenitor cells, endothelial cells and hematopoietic progenitor cells; 2) At the molecular level, exploring the molecular mechanism of vitronectin and integrin αvβ3 in regulating hematopoietic differentiation of hiPSCs; 3) At the functional level, clarifying the effect of vitronectin and αvβ3 on the function of hematopoietic progenitor cells both in vitro and in vivo. Our study would lay a methodological and theoretical foundation for the exploration of normal hematopoietic development and blood cell therapy.