电针预处理经miR-34a-5p调节IL-1β/COX2/PGE2炎症通路预防偏头痛机制研究

针刺预防偏头痛疗效确切。IL-1β/COX2/PGE2炎症通路参与三叉神经血管系统激活。前期研究显示电针抑制偏头痛大鼠三叉神经节IL-1β/COX2/PGE2炎症通路。偏头痛发作时miR-34a-5p表达上调。miR-34a/SIRT1调节IL-1β表达。因此，我们提出“电针预处理抑制miR-34a-5p表达，上调SIRT1表达，进而抑制IL-1β/COX2/PGE2炎症通路，减少CGRP 释放，预防偏头痛发作”的工作假说。首先通过体外实验证实miR-34a-5p/SIRT1是否调控三叉神经节IL-1β/COX2/PGE2炎症通路，再以偏头痛大鼠模型为研究对象，验证电针预处理是否经miR-34a-5p/SIRT1抑制三叉神经节IL-1β/COX2/PGE2炎症通路，从而抑制三叉神经血管系统激活，论证上述假设是否存在。

Migraine is a neurovascular disorder with gastrointestinal and visual symptoms that affects about 15% of people worldwide. Acupuncture is effective for migraine prophylaxis and for management of acute migraine attacks, without the adverse secondary effects associated with medications. Activation of the trigeminovascular system plays an essential role in inducing pain during a migraine attack. IL-1β stimulates COX-2 dependent PGE2 synthesis and CGRP release in rat trigeminal ganglia cells. The IL-1β/COX2/PGE2 inflammatory pathway contributes to activation of the trigeminovascular system. Electroacupuncture pretreatment inhibits IL-1β/COX2/PGE2 inflammatory pathway in the trigeminal ganglion of rats with migraine, and electroacupuncture plays protective roles in some diseases. Migraine attacks are associated with an acute upregulation in miR-34a-5p expression. It had be found that miR-34a/SIRT1 modulates expression of IL-1β. There is a need to investigate whether electroacupuncture can prevent migraine attacks by increasing SIRT1 expression via down-regulating expression of miR-34a-5p in the trigeminal ganglion, which inhibits IL-1β/COX2/PGE2 inflammatory pathway. Firstly, the effect of miR-34a-5p/SIRT1 on IL-1β/COX2/PGE2 inflammatory pathway in the trigeminal ganglion should be investigated in vitro. Secondly, we would testify the hypothesis whether electroacupuncture can prevent migraine attacks through miR-34a-5p/SIRT1 regulating IL-1β/COX2/PGE2 inflammatory pathway in the trigeminal ganglion in vivo.