申请人对广泛种植的冷季型草坪草高羊茅进行研究后发现，耐荫性较强的高羊茅品种Arid3在受到遮荫胁迫时，一氧化氮合酶（NOS）活性得以增强，同时，信号分子一氧化氮（NO）和脱落酸（ABA）的含量升高。为进一步揭示高羊茅Arid3的耐荫机制，我们通过电子克隆和RACE技术，从高羊茅叶片中克隆出与NO合成相关的NOS蛋白基因FaNOA1。在此基础上，本课题拟通过实时定量PCR检测FaNOA1基因在遮荫胁迫下的时空表达变化，同时利用蛋白质免疫印迹法（Western blotting）分析ABA对NOS蛋白表达的影响，验证ABA通过NO信号途径对高羊茅耐荫性的调控机理，明确遮荫下ABA和NO信号分子的转导机制，揭示ABA诱导耐荫相关基因FaNOA1的表达与耐荫性之间的关系。该研究结果将为耐荫草坪草新品种的分子设计、育种和遗传改良提供理论依据。

In our previous studies, we found that nitric oxide synthase (NOS) activity, nitric oxide (NO) and abscisic acid (ABA) content increased in the more shade-tolerant Arid3 of tall fescue, a widely used cold-season turf grass, under shade stress. To further explore the mechanism shade of tall fescue Arid3, the cDNA of FaNOA1 associated with NO synthesis was cloned by silico cloning and RACE technology from Arid3 of tall fescue. On this basis, The objective of this study is to elucidate the regulation mechanism of ABA signaling pathway via NO in shade tolerance of tall fescue, to clarify the signal transduction mechanisms of ABA and NO under shade stress, and to reveal the relationship between the expression of FaNOA1 induced by ABA and shade tolerant, by real-time PCR to detect changes in the expression of genes FaNOA1 and by Western blotting to analyze the effect of ABA on the expression of NOS protein. These results will provide theoretical basis for the molecular design, breeding and genetic improvement of new varieties of shade-tolerant turf grass.