糖尿病性溃疡是糖尿病严重的并发症之一，临床上易诊断难治疗，其发病机制尚不明确，有研究表明该病的发生可能与其创面组织中晚期糖基化终末产物(AGEs)的异常蓄积密切相关，但AGEs在糖尿病性溃疡组织中的表达情况及其在糖尿病性溃疡发病中的具体作用机制尚未见报道，我们推测AGEs-RAGE信号转导通路在其愈合过程中可能发挥关键作用。本项目以AGEs为切入点，通过建立糖尿病大鼠溃疡模型，应用ELISA、Western Blot、RT-PCR及免疫组化等先进技术，明确AGEs及其信号转导通路在糖尿病性溃疡创面愈合过程中的表达，阐明其具体作用机制，并通过湿润烧伤膏（MEBO）的干预研究，探讨MEBO促进糖尿病性溃疡创面修复及组织再生的药物作用靶点，为中医药"祛瘀生肌、扶正补虚"防治糖尿病性溃疡提供理论依据和可资借鉴的研究思路。

Diabetic ulcer is one of the serious compliactions of dibetes. It is easy to diagnose but hard to be cured.With unclear patheogensis, diabetic ulcer is regarded to be closely related with abnormal accumulation of AGEs in skin tissure, especially with AGEs-RAGE signal transduction pathway which plays a key role on wound healing.while few researches about the expression and mechanism of AGEs in Diabetic Ulcer were reported. We suspect that AGEs-RAGE signal transduction pathway plays a key role on wound healing.This program took AGEs-RAGE signal transduction pathway as a starting point,established the diabetic ulcer model on the diabetic rats and applied ELISA, Western Blot, RT-PCR and immunohistochemistry to explain how MEBO treament promote wound healing of diabetic ulcer from the the prospetive of molecular and cellular signal transduction, providing a new research method and basis for the TCM theory of eliminating necrotic tissues,promoting granulation and strength the vital to prevent and cure the diabetic ulcer.