白细胞介素-18在瓣膜内出血促进主动脉瓣钙化中的作用

随着社会老龄化，钙化性主动脉瓣狭窄发病率逐年增加。瓣膜内出血促进主动脉瓣钙化，而炎症在这个病理过程中的作用研究较少。本研究拟从1.评估钙化和非钙化主动脉瓣标本的铁代谢产物和IL-18表达的相关性；2.以白细胞介素-18与铁代谢相关蛋白的相互作用为切入点，在更接近实际病理状态的体外培养条件下，阐明瓣膜内出血促进主动脉瓣钙化中IL-18的作用；3.通过基因敲除小鼠主动脉瓣钙化动物模型，观察不同条件下体内瓣膜钙化情况，进一步证实体外研究结果。本研究的完成为延缓主动脉瓣钙化进展提供科学依据，为其他心血管钙化疾病的防止提供新的思路和靶点。

With the aging of society, the incidence of calcific aortic valve disease is increasing year by year. Intraleaflet haemorrhage contributes to aortic valve calcification, and the role of inflammation in this pathological process is less. The purpose of this study was to elucidate the role of IL-18 in the promotion of intraleaflet haemorrhage to aortic valve calcification through clinical specimens, in vitro culture and animal models. The completion of this study provides a scientific basis for delaying the progression of aortic valve calcification, and provides new ideas and targets for the prevention of other cardiovascular calcification.