蜕膜间质细胞旁分泌的EGF通过诱导上皮间质转化促进滋养细胞侵袭和迁移的研究

胚胎植入子宫内膜是妊娠建立的初始过程和胚胎持续发育的关键，也是生殖医学研究的热点。在前期工作中我们发现与子宫内膜间质细胞相比，早孕期蜕膜间质细胞条件培养基能显著促进滋养细胞的侵袭和迁移。进一步的结果还提示滋养细胞上皮间质转化（EMT）可能参与此过程，而EGF可能是蜕膜间质细胞分泌的参与调节滋养细胞EMT转化的活性细胞因子。本项目通过对蜕膜间质细胞诱导滋养细胞EMT转化对细胞侵袭和迁移的影响及旁分泌EGF调节细胞内通路激活影响滋养细胞EMT的胞内分子调节过程及机制的研究，进一步了解滋养细胞与蜕膜细胞之间的分子对话，尤其是蜕膜间质细胞通过分泌EGF诱导滋养细胞发生EMT转化的作用机制，对完整阐述胚胎植入和妊娠维持过程中母胎界面细胞和分子生物学活动具有重要意义。

The trophoblasts invading into the maternal endometrium is the key point to establish the process of pregnancy and sustainable embryo development, which is also one of the hot research area of reproductive medicine. Our previous studies found that, compared to the endometrial stromal cells, decidual stromal cells(DSC) promoted trophoblast invasion and migration. Further studies demonstrated that thophoblastic epithelial mesenchymal transition (EMT)may involve in the process and EGF may be the active cytokines secreted by DSC in regulation of trophoblastic EMT. This project is planned to investigate the effect of DSC on trophoblast invasion and migration induced by EMT and the effect of EGF secreted by DSC on trophoblast EMT and its intracellular pathways. This results will help to further understand the molecular dialogue between DSC and trophoblasts, especially the mechanism of DSC inducing EMT throught secretion of EGF to promote trophoblast invasion and migration, which is of great significance to completely illustrate the cell and molecular activity at the maternal-fetal interface during the process of embryo implantation and maintenance of pregnancy.