基于NF-κB/STAT3信号通路研究半夏泻心汤对胃癌前病变"微环境调和"的作用机制

胃癌前病变（PLGC）通常是炎症因子（如TNF-α、IL-1β）等多因素广泛参与，NF-κB/STAT3信号通路活化而致"微环境稳态" 失常的结果。经典方剂半夏泻心汤具有"调寒热、调气机、调虚实"的功效，研究证实半夏泻心汤具有较好的逆转PLGC组织病理学及保护粘膜作用。其是否具有"调整调和"胃粘膜微环境稳态作用，从而达到逆转PLGC效应并不清楚。本项目拟在整体、细胞、分子水平上研究半夏泻心汤作用于PLGC 大鼠NF-κB/STAT3信号通路等引起的"胃粘膜微环境失稳"三个关键环节级联反应的效应，验证假说。揭示NF-κB/STAT3信号通路活化在半夏泻心汤证胃粘膜微环境失稳过程中的关键作用以及各因素相互作用的实质。从多层次分析半夏泻心汤证的"胃粘膜微环境"特点，及半夏泻心汤对"胃粘膜微环境"的调和作用机制。

Gastric precancerous lesions (PLGC) is usually inflammation factors (such as the TNF alpha, IL-1 beta) many factors such as the broad participation, the nf-kappa B/STAT3 signal pathway activation and the "micro environmental stabilization" wrong results. Classical prescription "banxia xiexin decoction" with "the cold, the angry machine, the balance is the effect, study confirms" banxia xiexin decoction "has good reversal PLGC histopathological and protect mucous membrane function. If it has "adjust harmonic" gastric mucous membrane micro environmental steady-state function, so as to achieve the reversal PLGC effect is not clear. This project is in overall, cells, and the molecular level research "banxia xiexin decoction" in the role of PLGC rat the nf-kappa B/STAT3 signal pathway by "gastric mucous membrane micro environmental instability" three key link cascade effect, testing hypotheses. Reveals the nf-kappa B/STAT3 signal pathway activation in "banxia xiexin decoction" card gastric mucous membrane micro environmental instability process critical role as well as the factors of the interaction of the essence. Multi-layer analysis from "banxia xiexin decoction" card "gastric mucous membrane microenvironment" features "banxia xiexin decoction" and to "gastric mucous membrane microenvironment" harmonic function mechanism.