热休克蛋白在温热诱导皮肤免疫中的作用及调控研究

前期临床研究发现，一定温度范围的温热可以通过诱导机体建立抗病毒免疫而治愈多发、难治性人乳头瘤病毒（human papilloma virus，HPV）感染性疾病。热休克蛋白（heat shock protein，HSP）为一组应激反应性蛋白，一定条件下参与免疫应答反应的建立。本研究拟采用温热处理皮肤组织和不同皮肤细胞（正常及HPV感染状态），高通量检测多种热休克蛋白分子表达状态，并获取特异表达的热休克蛋白分子所结合的功能蛋白；进一步检测特异表达的热休克蛋白对皮肤朗格汉斯细胞免疫功能的影响及其转录和转录后水平调控机制，探索温热诱导（或抑制）机体免疫的机制。本研究将为基于温热的疫苗开发或建立人工诱导免疫治疗肿瘤提供实验依据。

Previously, we observed clinically that human papilloma virus infected diseases could be cleared by hyperthermia-induced systemic immunity. To investigate the mechanism beneath the interesting phenomena, the hyperthermia is introduced to mouse skin tissue in vivo and different skin cells in vitro, in which differentially expressed heat shock proteins (HSP) and chaperons are identified by high through put HSP PCR array. Then effects of these HSP on Langerhans cell functions and related mechanisms at transcriptional and post transcriptional levels are investigated. This study could contribute to further understanding of the role of HSP and Langerhans cell in skin immune system, and accordingly, to establish artificially induced anti-tumor immune or vaccines, or to induce immune tolerance to treat allergic diseases and promote skin transplantation.