细胞核/叶绿体之间的信号交流对于高等植物叶绿体发育、细胞核/叶绿体基因组协同表达以及光合作用调控至关重要。VAR6/PAC是细胞核基因组编码、定位于叶绿体、参与叶绿体基因组表达的必需蛋白，其低表达突变体var6-1初生真叶白化，光合作用相关蛋白亚基稳态积累量严重下降。针对var6-1的抑制突变体基因克隆及回复验证实验表明，抑制突变体S04-03和S05-31A能够抑制var6-1的表型。S04-03和S05-31A分别编码核孔蛋白Nup160和Nup96，并属于Nup107-Nup160亚复合体。这些为核孔复合体调控细胞核/叶绿体信号交流提供了重要遗传学证据。本研究拟通过细胞生物学、转录组学和蛋白组学层次的研究，探索核孔复合体突变对于细胞核/叶绿体之间的信号交流分子在细胞核积累的影响，最终建立核孔复合体调控细胞核/叶绿体信号交流的分子模型。

Signal communication between nucleus and chloroplasts are necessary for biogenesis of chloroplasts, synergetic expression between nucleus genome and chloroplast genomes, as well as regulation to photosynthesis. VAR6/PAC is one of the essential chloroplast localized factors, encoded by nucleus genome, to regulate expression of chloroplast genomes. var6-1 is the VAR6/PAC knock-down mutant with albino ture leaves at early stage, and with decreased accumulation of photosynthetic subunits. Suppressor mutants of var6-1 were screened defined by green true leaves and also recovery accumulation of photosynthetic subunits. The locus of S04-03 suppressor and the S05-31A suppressor was determined as Nup160 and Nup96 respectively, by map based cloning and complementation assay. Both Nup160 and Nup96 are nucleoporins of the Nup107-Nup160 subcomplex belonging to nuclear pore complex (NPC). These are genetic evidences that NPC regulates signal communication between nucleus and chloroplasts. In this research, we are going to discover the accumulation of signal molecules in the nucleus of NPC mutants with cell biology combined transcriptome and proteome strategies, to finally establish the model of signal communication between nucleus and chloroplasts regulated by NPC.