大豆分枝数新QTL位点qBN-18的图位克隆及功能解析

分枝数是影响大豆产量的重要因素之一，挖掘调控大豆分枝发育的QTL/基因对提高大豆产量、培育理想株型的大豆品种具有重要意义。申请者前期利用多分枝和少分枝大豆构建的F2、RIL群体以及回交群体，将大豆分枝数新QTL位点qBN-18定位在63.7Kb区间，包含9个注释基因。本研究拟在前期工作基础上，利用不同定位群体，综合重组个体筛选、定位区间基因序列分析和表达分析等方法，图位克隆qBN-18基因。通过遗传转化和功能研究、基因表达以及蛋白的特性研究，解析qBN-18的生物学功能。通过与其他分枝发育相关基因调控关系研究，深入研究qBN-18在调控大豆分枝发育的机理。同时，通过近等基因系的筛选和鉴定，研究大豆分枝数与种植密度、产量等的关系，开发功能性分子标记并明确标记的鉴定效率，为分子标记辅助的大豆株型育种提供指导。

Branching is an important trait influencing soybean yield. Furthermore, branching number and distribution affect plant density, lodging resistance, and photosynthesis. In our previous study, a new QTL qBN-18 related to branch number of soybean was identified from a population developed from Kennong 24 and Kenfeng 19. qBN-18 was further fine-mapped to a region of 63.7kb, containing nine genes in the reference genome. In this project, we are intend to clone qBN-18 by map based cloning method. The biological function of qBN-18 will be carefully investigated by genetic transformation, gene expression, and protein analysis. Meanwhile, the molecular mechanism of qBN-18 in regulating branch development will also be revealed. In addition, the relationship of this gene with plant density and soybean yield will also be studied. Functional markers will be evaluated for marker assisted selection in breeding program. The results of this study will provide guidance for mechanisms of branch development and plant architecture breeding practice.