基于边缘系统-下丘脑-垂体-肾上腺轴途径探讨隔药饼灸对功能性消化不良胃肠动力的调节机制

功能性消化不良（FD）是持续或反复发作的餐后上腹饱胀、早饱、上腹痛、上腹烧灼感而没有器质性病变的功能性疾病，与心理精神因素、胃肠动力障碍密切相关，已成为困扰人们生活和工作的一大疾病，针灸疗法对其有着很好的疗效。研究表明，边缘系统-下丘脑-垂体=肾上腺轴（LHPA轴）在FD的发病机制中发挥着重要的作用，对胃肠运动的调节有着重要的意义。我们在前期研究的基础上提出：用隔药饼灸治疗功能性消化不良胃肠动力障碍，是否是通过LHPA轴来进行良性平衡调节而实现的呢？本课题旨以LHPA轴为切入点，以激素、神经递质和受体及基因表达的变化量为中心，以FD肝郁脾虚模型大鼠为受试对象，运用HPLC-ED、RIA、qRT-PCR、Western Blot等多种技术手段，研究隔药饼灸对FD肝郁脾虚模型大鼠行为学、胃排空、小肠推进、胃电图、激素水平、神经递质和受体及基因表达量的影响，探讨隔药饼灸治疗FD可能的作用机制。

Functional dyspepsia（FD） is the functional disease which refers to the continuous or repeated attacks on postprandial epigastric full bilge unwell, early satiety, abdominal pain, burning sensation in the upper abdomen and no organic disease, FD is closely related to the psychological mental factors and gastrointestinal motility disorder, and it has become a disease which was bothered the people's life and work , acupuncture and moxibustion treatment for it has good curative effect. Research shows, the limbic system-Hypothalamic-pituitary-adrenal axis (LHPA) plays an important role in the pathogenesis of FD ,and that has great significance in the regulation of Gastrointestinal movement.Based on the previous studies we propose a hypothesis: using the treatment of Herbal-cake-separated moxibustion on functional dyspepsia gastrointestinal motility disorders, whether it was through the limbic system - Hypothalamus - pituitary - adrenal axis for benign balance regulation to achieve the therapeutic effect? This subject is from the LHPA axis as the breakthrough point, hormones, neurotransmitter and receptor and related gene expression change as the center, FD rats model with syndrome of liver stagnation and spleen deficiency for subjects, using many kinds of technical means such as HPLC-ED, RIA, qRT-PCR, Western Blot and so on ,to study the influence on FD model rats behavior, Gastric emptying、small intestinal propulsion、electrogastrogram, hormone level, neurotransmitter and receptor and related gene expression quantity with Herbal-cake-separated-moxibustion, to explore the possible mechanism of action.