马铃薯StSABP2和StSAMT介导SA-MeSA稳态在早疫病抗性中的作用及其调控机制

水杨酸（SA）和水杨酸甲酯（MeSA）在植物体内通过SABP2和SAMT的介导可以进行相互转化而形成动态平衡（SA-MeSA稳态），对植物防御反应至关重要。申请人前期研究发现StSABP2和StSAMT在外源SA和MeSA介导的马铃薯早疫病抗性中受到诱导表达，推测它们很可能通过调控SA-MeSA稳态在马铃薯抗早疫病中发挥重要作用。因此，本项目拟利用遗传学、生理学及分子生物学技术，进一步研究StSABP2和StSAMT突变体及过表达植株在早疫病菌胁迫下不同时间点的StSABP2和StSAMT表达特性对SA-MeSA稳态的调控作用，系统解析SA-MeSA稳态与马铃薯早疫病抗性反应之间的相关性，阐明StSABP2和StSAMT介导SA-MeSA稳态在早疫病抗性中的作用及调控机制。本研究将为创制抗早疫病马铃薯新种质提供实验证据和遗传材料，对有效开展马铃薯抗早疫病分子设计育种具有重要指导意义。

Salicylic acid (SA) and methyl salicylate (MeSA) can mutually transform under the regulation of SABP2 and SAMT to further form SA-MeSA homeostasis in plants, which is essential for plant defense responses. We have found that the expression of StSABP2 and StSAMT were induced in potato early blight resistance mediated by exogenous SA and MeSA, and it is speculated that StSABP2 and StSAMT might play an important role in potato resistance to early blight by mediating SA-MeSA homeostasis. Therefore, in this project, the regulation of SA-MeSA homeostasis by the expression characteristics of StSABP2 and StSAMT at different time points in StSABP2 and StSAMT mutants and overexpressing plants under the stress of Alternaria solani was intended to be studied via genetics, physiology and molecular biology techniques. Meanwhile, the correlation between SA-MeSA homeostasis and early blight resistance in potato was systematically analyzed to clarify the role and regulation mechanism of SA-MeSA homeostasis mediated by StSABP2 and StSAMT in early blight resistance. This study will provide experimental evidence and genetic material for the creation of new potato germplasms resistant to early blight, and will have important guiding significance for effective development of molecular design breeding of resistance to early blight in potato.