植物自噬基因 ATG1 启动细胞自噬的调控网络

自噬是细胞内物质周转的主要途径，参与到了植物生长发育、响应环境等过程中。由于其重要作用，自噬已成为植物领域的一个研究热点。随着研究的不断深入，多个植物自噬相关基因(AuTophaGy, ATG)被陆续鉴定，它们相互协作，以复合体的形式参与了细胞自噬的起始、成核、囊泡扩展与闭合、运至液泡降解等过程中。目前，对植物自噬起始的调控机制还不清楚。为了解析起始机制，需要从负责自噬起始的ATG1/13蛋白激酶复合体开始研究。ATG1作为自噬蛋白中唯一的激酶，在接收到环境信号之后，是通过磷酸化调控哪些下游靶蛋白，以及如何调控靶蛋白来起始自噬通路的？为了解决这个科学问题，我们拟在基于磷酸化蛋白质组分析和邻近标记技术的基础上，筛选ATG1可能的下游靶蛋白，并对其进行功能验证，从而深入解析植物自噬基因 ATG1 启动细胞自噬的调控网络。

As the main turnover pathway of intracellular material, autophagy does involve in plant growth and development, responding to the environment and other processes. Autophagy has become a hotspot in the field of plant research because of its critical role. Multiple Autophagy related genes (ATG) have been identified. They cooperate with each other and participate in the initiation, nucleation, expansion and closure of vesicles, transporting to vacuolar and other processes in autophagy in the form of complexes. At present, the regulatory mechanism of autophagy initiation in plants is still unclear. To elucidate the initiation mechanism, we need to study the ATG1/13 protein kinase complex, which is responsible for initiation of autophagy. As the only kinase of ATG proteins, how does ATG1 regulate target proteins to initiate autophagy pathway after receiving environmental signals? In order to solve this scientific problem, we plan to screen possible downstream target proteins of ATG1 based on phosphoproteome analysis and Proximity labeling technique, and verify their functions, so as to further analyze the regulatory network of ATG1.