Aurora激酶A(Aurora-A)是一种参与细胞有丝分裂调控的丝氨酸/苏氨酸激酶。新近研究表明Aurora-A参与了卵母细胞减数分裂的调控事件，但有关该激酶参与猪早期胚胎首次有丝分裂的调控目前仍不十分清楚。本研究拟采用实时荧光定量PCR、免疫印迹和间接免疫荧光等方法检测Aurora-A在猪体外受精胚胎（IVF）与体细胞克隆胚胎(SCNT)胚胎首次卵裂过程中的动态表达规律及其亚细胞定位；并通过RNA干扰与mRNA显微注射研究Aurora-A表达抑制或过表达对猪胚胎首次有丝分裂过程的影响；最后通过特异性抑制试验，分析Aurora-A是否参与抑制丝裂原激活蛋白激酶(MAPK)信号通路的调控，探讨Aurora-A调节猪胚胎有丝分裂的分子机制。以期揭示Aurora-A在猪早期胚胎，特别是克隆胚胎首次有丝分裂过程中的功能及其分子调控机制，为深入研究猪早期胚胎发育机制提供实验参考。

Aurora kinase A (Aurora-A) is a serine/threonine kinase that functions in various stages of mitosis. Accumulating evidence has demonstrated that gene amplification and overexpression of Aurora-A are linked to tumorigenesis and increasingly recognized in cancer research. In recent years, Aurora-A has been shown to significantly involved in the regulation of oocytes meiosis, however, its roles in porcine early embryos development is still unclear, especially in porcine cloned embryos. Quantitative real-time RT PCR and Western blotting will be performed to analyze the expression of Aurora-A mRNAs and proteins, and the subcellular localization of Aurora-A proteins will also be determined by confocal microscopy. To investigate the significance function of Aurora-A in porcine early embryonic development, the expression of Aurora-A will be knocked down or overexpressed by microinjection of Aurora-A shRNA plasmid or Aurora-A mRNA．We will detect the expression of Aurora-A and its kinase activity changes after inhibition of mitogen-activated protein kinase(MAPK)signaling．The aim of this study is to determine the effects of porcine Aurora-A on the first mitosis of porcine IVF and SCNT embryos, so as to establish a basis for the further research on the development mechanism of porcine early embryos.