前期研究采用基因芯片检测发现长链非编码RNA（lncRNA）BMP2-1和mRNA 骨形成发生蛋白2（BMP2）在成骨诱导的人牙周膜干细胞（hPDLSCs）中显著上调表达，PCR验证显示两者表达趋势一致；两者位置毗邻且共表达分析提示两者之间存在显著正相关；抑制lncRNA BMP2-1表达后BMP2在基因和蛋白水平均显著下降。据此我们推测，lncRNA BMP2-1可能通过靶向正调控BMP2促进hPDLSCs的成骨分化。本课题拟构建lncRNA BMP2-1过/抑制表达的稳定细胞系，分析lncRNA BMP2-1对成骨分化的影响；观察lncRNA BMP2-1的细胞内定位，明确其调控的靶基因及作用机制；进一步探索lncRNA BMP2-1通过调控BMP2可能参与的信号通路。研究旨在阐明lncRNA BMP2-1调控hPDLSCs成骨分化的功能及机制，为牙周再生提供新思路。

Using microarrays, our previous work have detected the changes of long non-coding RNA (lncRNA) and mRNA in human periodontal ligament stem cell (hPDLSCs) before and after osteogenic induction in the previous work. The results showed that lncRNA BMP2-1 and mRNA bone morphogenetic proteins 2 (BMP2) were both significantly up-regulated after the induction, and the coding-non-coding gene co-expression analysis also indicated their significant positive correlation. And bioinformatics analysis showed their close location. The mRNA and protein level of BMP2 decreased significantly after down-regulated lncRNA BMP2-1. Therefore, we hypothesized that lncRNA BMP2-1 might be involved in osteogenic differentiation of hPDLSCs by regulating mRNA BMP2. The present project will try to establish cell line of gene silencing of lncRNA BMP2-1, to study the impact of lncRNA BMP2-1 on the osteogenic differentiation of hPDLSCs in vivo and in vitro, to observe subcellular distribution of lncRNA BMP2-1, to investigate the interactions between mRNA BMP2 and lncRNA BMP2-1. The objective of this project is to elucidate the effects and mechanism of involvement of lncRNA BMP2-1 in the osteogenic differentiation of hPDLSCs by regulating mRNA BMP2. This study would be significant for revealing the important role of lncRNA in the reconstruction of periodontal tissue defects, and it also provides a new idea for periodontal tissue regeneration in the future.