我们前期在骨髓增生异常综合征(MDS)难治性贫血伴原始细胞增多(RAEB)亚型中同时进行了长链非编码RNA(lncRNA)和mRNA芯片检测，通过生物信息学分析构建了MDS发病调控网络图，发现5条与Wnt/β-catenin信号通路相关的lncRNA。为了研究哪些lncRNA对Wnt通路有调控作用以及具体的调控机制，构建lncRNA沉默和过表达细胞模型，采用qRT-PCR、Western blot等方法选择对Wnt通路活性和对MDS肿瘤细胞增殖生长影响最大的lncRNA作为研究调控机制的目标lncRNA。进一步用荧光素酶报告基因、RNA pull-down、RNA结合蛋白免疫沉淀研究该lncRNA调控Wnt通路的机制：包括作用途径、靶点、lncRNA是否作为miRNA-96的前体参与调控、lncRNA与micRNA是否直接作用等。该研究对阐明MDS-RAEB亚型的发病机制有重要的意义。

We have completed the microarray detection of long noncoding RNA (lncRNA) and mRNA in cases with myelodysplastic syndromes (MDS)-refractory anemia with excess blasts (RAEB) subtype. A regulation network of lncRNA-microRNA-mRNA-transcription factor was built using bioinformatics approach. In this network, we found 5 lncRNAs were related to Wnt/β-catenin signal pathway.Therefore, we consider that there are certain lnRNAs which may regulate the Wnt signal pathway and play important roles in the disease progression of MDS. We will construct knock-out and over-expressed models for lnRNA, and use qRT-PCR, Western Blot, CCK-8 and flow cytometry approaches to verify the role of predicted lncRNA in Wnt signal pathway. We need to find the target genes, and verify the controlling mechanism of predicted lncRNA in Wnt pathway by fluorescence reporter assays, RNA pull-down and RNA binding protein precipitation experiment. Our study will be of very significant in explaining themechanism of MDS disease progression.