新型红光碳点用于线粒体内金属离子的传感与成像分析

设计和开发线粒体靶向荧光探针，对于线粒体结构相关的细胞生理和病理过程的探究具有重要意义。本研究以红光碳点为荧光探针，三苯基膦为线粒体靶向基团，通过金属识别基团的引入，构建了基于线粒体内金属离子检测的新型荧光探针。首先通过可控制备得到具有红光或近红外发光性能的碳点。然后基于荧光/电子共振能量转移原理，通过引入特异性识别基团，实现对线粒体内金属离子的高选择性成像分析。据此建立的新型荧光传感平台，一方面可实现实际样品中待测物的高特异性和高灵敏检测，另一方面可实现线粒体内金属元素的高分辨可视化成像分析，减少生物体自体荧光干扰，并达到实时监控线粒体内金属离子的浓度变化的目的。本项目的开展将有力拓展碳点在细胞成像与传感领域的应用，克服传统荧光探针背景信号强、易受复杂环境干扰的缺陷，为设计新型探针应用于复杂体系的检测与分析提供新思路。

The development of highly specific mitochondrial-targeted fluorescence probe is of great significance to study the physiological and pathological processes related to the mitochondrial. And there is great desire to develop fluorescent probes that can sensitively and selectively detect metal ions in mitochondria. In this study, the red emissive carbon dots are employed as fluorescent probes, and it could be covalently conjugated with a mitochondria-targeting moiety, i.e. triphenylphosphonium (TPP). By introducing metal recognition groups, a new fluorescent sensing nanoplatform based on the detection of metal ions in mitochondria is constructed. In this system, red emissive carbon dots can be obtained. Via the mechanism of fluorescence/electron resonance energy transfer, the nanoprobe exhibit relatively high sensitivity and selectivity towards metal ions. The as-prepared new fluorescence sensing platform, allowing labeling and imaging of the related metal ions in mitochondria in real time, and reducing organism autofluorescence interference. The development of this project will board the application of carbon dots in cell imaging and sensing, overcome the background signal and complex environmental interference of traditional fluorescent probe, and provide new ideas for designing new probes to detect and analyze complex systems.