潜水人群铜绿假单胞菌职业感染高发,其机制至今不清，las/rhl系统是其临床毒力调节的重要通路。本研究组前期发现氦氧饱和高气压暴露明显诱导las/rhl基因和毒力基因表达，但其诱导las/rhl通路介导铜绿假单胞菌毒力调节机制仍空白。本项目首先研究长期潜水环境职业暴露对野生型菌株las/rhl基因序列SNPs型和mRNA水平及细菌毒力表型的调节影响，查找职业暴露对菌株生物学胁迫效应的分子基础；然后实验模拟暴露，应用"功能分类基因芯片"技术,研究正常菌和QS基因功能缺陷菌las/rhl上、下游调节通路基因及细菌毒力基因的表达变化，结合暴露细菌las/rhl 基因序列SNPs分析、信号分子AHLs检测及其毒力表型检测，分析las/rhl通路调控介导能力与细菌毒力调节的关系。研究结果能揭示暴露环境诱导las/rhl系统介导毒力调节的机制。可为控制潜水职业性感染高发的新靶点研究提供新理论

Pseudomonas aeruginosa is a kind of microbe with high infectivity, but the mechanism of infection in saturation diving is unclear till now. Las/rhl system has been kown as the notable regulatory pathway on the virulence adaptative of P. aeruginosa in clinic infection. Our previous experiments indicate that hyperbaric oxyhelium exposure remarkably induces the expression of las/rhl system genes and several virulence genes. It is still, however, unknown how the las/rhl system mediate the inducing of the bacterial virulence adaptation under the hyperbaric oxyhelium exposure. In this project, the molecular evolution of wild type P. aeruginosa isolated from our naval professional divers was firstly examined by detecting the SNPs, the mRNA of las/rhl and the virulence genotype as well as phenotype of P. aeruginosa under the stress on long-time exposure of professional saturation diving environments. Under imitation hyperbaric oxyhelium in laboratory condition, the focused DNA microarray technology was then used to study the gene expression pattern of las/rhl regulatory pathway and the virulence factors in both normal strains and the QS gene functional defected strains. Finally, the relationship between las/rhl pathyway and the induced virulence adaptative of P. aeruginosa under imitation hyperbaric oxyhelium environment will also be further established with the detection of SNPs, mRNA of las/rhl, virulence factors' genotype and phenotype in both strains. These results will elucidate our hypothesis that las/rhl pathyway plays a key role in the regulation of the high infectivity of P. aeruginosa in professional saturation divers.