2010年以来，坦布苏病毒在我国多省暴发和流行，对水禽养殖业造成巨大损失。本课题组前期工作显示大肠杆菌表达的坦布苏病毒E蛋白结构域I/II蛋白对病毒感染具有抑制作用。由于重组蛋白分子量较大，结构复杂，因此可能存在不利于发挥抑制活性的区域。为了进一步定位E蛋白结构域I/II中抑制病毒感染的核心序列，本项目设计并合成覆盖坦布苏病毒E蛋白结构域I/II并相互重叠的多肽；利用细胞感染抑制试验，鉴定具有抑制作用的多肽（抑制肽）；在此基础上，分析抑制肽的抗病毒特性；最后，研究抑制肽对病毒粒子结构、病毒与细胞受体结合及病毒膜融合的影响，明确其抑制病毒入侵的作用机制。本研究旨在为抗坦布苏病毒药物的设计提供新的研究靶点，并为进一步预防和控制坦布苏病毒病奠定理论基础。

Huge losses have been caused to the breeding industry of waterfowl by the outbreak and spread of tembusu virus in several provinces since 2010. It’s found that purified E protein domains I and II of tembusu virus expressed in E. coli was able to inhibit virus infection. Because of the high molecular weight and complex structure of the recombinant protein, there may be regions that is unfavorable to exert inhibition. In order to identify the core inhibitory sequences, overlapping peptides covering tembusu virus E protein domains I and II are designed and synthesized artificially; and then, cell infection tests are used to define the peptides which inhibited tembusu virus infectivity (peptide inhibitor); on this basis, antiviral characteristics of peptide inhibitors are analyzed; finally, the inhibition mechanism of peptide inhibitor is clarified by analyzing the impacts of peptides on structure of viron, binding between virus and its receptor, and virus membrane fusion during viral infection. This study will provide a new target for the design of anti-tembusu virus drugs and lay the theoretical foundation for prevention and control of diseases caused by tembusu virus.