长链非编码RNA（lncRNA）作为一种新发现的表观遗传调控因子，在肿瘤的发生发展中发挥重要作用。目前对lncRNA在胰腺癌中的作用及相关分子机制还缺乏深入研究。我们前期研究通过lncRNA芯片发现一种在胰腺癌组织中高表达的CRNDE，同时还发现CRNDE的表达与LATS1分子表达水平密切相关。LATS1是Hippo-YAP通路的关键分子，而且在其启动子区含有甲基化位点。我们推测CRNDE可能通过调控LATS1表达介导Hippo-YAP通路在胰腺癌发生发展中发挥作用，但具体分子机制尚不明确。本课题首先拟明确CRNDE在胰腺癌组织中的表达及临床意义；然后在细胞与动物水平研究CRNDE对胰腺癌细胞增殖、凋亡与侵袭的影响；最后探讨CRNDE通过甲基化修饰调控LATS1表达，介导Hippo-YAP通路在胰腺癌细胞恶性转化中的分子作用机制。本研究将为深入探索胰腺癌发病机理和寻找新的治疗靶点奠定基础。

Long noncoding RNA (lncRNA) is a newly discovered epigenetic regulator and plays an important role in tumor development. However, the role of lncRNA in pancreatic cancer is still lack of awareness. Our previous study found a novel lncRNA CRNDE overexpressed in pancreatic cancer by lncRNA microarray assay, and the level of CRNDE expression was closely related to LATS1 expression. LATS1 is a key member of Hippo-YAP pathway, and has methylation sites in the promoter region. Therefore, we hypothesized that CRNDE regulated LATS1 expression, and played an important role in pancreatic cancer development through Hippo-YAP pathway, but its mechanism was still not clear. This paper intended to firstly study CRNDE expression in pancreatic cancer and its clinical significance; and then to study the effects of CRNDE expression on pancreatic cancer cell proliferation, apoptosis and invasion in vitro and in vivo; finally, to reveal the molecular mechanism of CRNDE regulates LATS1 expression by methylation in the malignant transformation of pancreatic cancer cells through Hippo-YAP pathway. And these will provide a theoretical foundation to further explore the mechanism of pancreatic cancer progression and search for new therapeutic targets.