克隆胚胎顺利发育的前提是去核的卵母细胞能够使供体细胞核的表观遗传修饰模式由分化状态转变为胚胎多能性状态。研究表明，母源重编程因子能够调控克隆胚胎表观遗传修饰模式重建，决定了克隆胚胎发育潜能。但目前对母源重编程因子调控克隆胚胎发育机制的认识还远远不够，而且也没有研究针对具有不同克隆胚胎发育潜能的卵母细胞进行重编程因子挖掘及阐明其作用机制。据此，本研究拟在前期工作基础上采用单细胞转录组测序来挖掘并筛选真正与猪克隆胚胎发育相关的母源重编程因子，继而利用过表达和干扰技术来阐明新发现的候选重编程因子在克隆胚胎发育、表观遗传修饰模式重建、关键基因表达模式调控和DNA损伤反应过程中的作用，最终揭示新发现的重编程因子调控克隆胚胎发育潜能的分子机制。相信该课题的探讨和阐明不仅能够挖掘到新的决定克隆胚胎发育潜能的重编程因子，而且将会对体细胞核移植重编程的机理研究及其在畜牧业和再生医学中的应用起到重要推动作用。

The premise of normal development of cloned embryos is the successful transformation of epigenetic modification patterns from differentiated donor cell to pluripotent embryo driven by the enucleated oocyte. Studies have suggested that maternal reprogramming factors can regulate the reconstruction of epigenetic modification pattern in cloned embryos, determining the developmental competence of cloned embryos. However, the role of maternal reprogramming factors in the development of cloned embryos is still poorly studied, and no study has been carried out to explore maternal reprogramming factors according to the developmental competence of cloned embryos derived from these oocytes and elucidate their mechanism. Accordingly, on the basis of preliminary research, this study will apply single cell transcriptome sequencing to explore and screen maternal reprogramming factors really related to the development of porcine cloned embryos, further investigate the effects of overexpression or knockdown of newly discovered candidate reprogramming factors on the development of cloned embryos, epigenetic modification reconstruction, the regulation of key gene expression pattern and DNA damage response in cloned embryos, and finally uncover the molecular mechanism underlying the developmental competence of cloned embryos regulated by newly discovered reprogramming factors. The discussion and clarification of this topic will discover new reprogramming factors determining the developmental competence of cloned embryos and promote the development of the research in nuclear reprogramming induced by somatic cell nuclear transfer and the application of somatic cell nuclear transfer in livestock and regenerative medicine.