Nogo蛋白家族基因SNPs及拷贝数变异与鼻咽癌的分子机制研究

鼻咽癌(NPC)是严重危害我国中老年人的一种头颈部恶性肿瘤，然其病因和发病机制尚未完全阐明。Nogo蛋白家族基因是新近发现的可能是参与肿瘤发生的一种新基因，但他通过何种机制在肿瘤中起作用尚不清楚。截止目前未见该基因在鼻咽癌中的研究。在参与的已结题国家自然科学基金前期研究中，我们课题组已经对Nogo蛋白家族基因SNPs进行了检测。在已有的前期基础上，本研究拟联合使用SNPs和CNV 两个遗传学标记，采用PCR和DNA测序技术检测Nogo蛋白家族基因SNPs 和CNV突变与鼻咽癌的关系。用Real-time PCR和Western blot等技术分别检测Nogo基因mRNA和蛋白的表达情况。分析Nogo基因突变对mRNA表达丰度及Nogo蛋白表达水平的影响，探讨Nogo基因表达调控的功能，揭示Nogo基因在鼻咽癌发生、发展中的分子作用机制，从而为鼻咽癌的基因诊断和疾病预防奠定基础。

Nasopharyngeal carcinoma (NPC) is a head and neck malignant cancer which is seriously harm to middle age and old people health, however, its etiology and pathogenesis have not yet fully understood. Nogo protein family gene is a novel gene which may be involved in tumorigenesis, but its mechanism involved in the incidence of tumors is not clear. Up to date, no data to studies, have examined the association of the Nogo protein family gene with NPC. In the previous National Natural Science Foundation study, the single nucleotide polymorphisms (SNPs) of Nogo protein family gene were examined by the polymerase chain reaction-single base extension technique and DNA sequencing methods in case-control study populations. Based on the previous basis, in this study, we plan to examine the relationship between single nucleotide polymorphisms (SNPs) and copy number variation (CNV) of Nogo protein family gene and risk of NPC by the polymerase chain reaction and DNA sequencing methods. Additionally, the mRNA and protein expression of Nogo protein family gene in NPC were detected by Real-time PCR and Western blot techniques. To analysis association of mRNA and protein expression of Nogo gene with the gene mutations in NPC patients, and to investigate the function of Nogo gene expression regulation in patients with NPC. Reveal the molecular mechanism of Nogo protein family gene in occurrence and development of NPC, and provides the research foundation for the gene diagnosis and prevention of NPC.