马铃薯纺锤块茎类病毒（PSTVd）是马铃薯生产中的重要制约因子，前期研究发现，不同马铃薯品种受到PSTVd侵染后症状表达存在明显差别，引起这种差别的分子机理目前尚不清楚。本项目拟在前期基础上，围绕不同马铃薯品种对PSTVd侵染的症状差异这一问题，通过RNA-Seq技术获取不同马铃薯品种接种PSTVd前后的转录组数据，应用生物信息学方法明确接种前后变化显著的小分子RNA（sRNA）和mRNA，筛选并鉴定引起sRNA变化的差异表达基因；通过qRT-PCR检测比较不同品种在PSTVd侵染后基因变化差异，明确差异靶标基因；结合RNAi等技术验证相关靶标基因在PSTVd侵染马铃薯过程中的生物学功能，阐明不同马铃薯品种对PSTVd症状差异应答的分子机制，以期为PSTVd的综合防控和抗PSTVd育种提供科学依据。

Potato spindle tuber viroid (PSTVd) is an important restriction factor in potato production. Previous studies showed that there were obvious differences of symptom among the different potato varieties infected by PSTVd, and the mechanism of the cause of the differences was not clear. This project aims to screen and identify differential expressed genes by obtaining transcriptome data using RNA-Seq technique and by confirming sRNA changes before and after inoculated by PSTVd with bioinformatics method. Differential target genes will be clarified by comparing differences of the gene changes in different potato varieties using qRT-PCR technique. Biological functions of relevant target genes during PSTVd infection will be verified by RNAi technique in order to explain the mechanism of differential responses among different potato varieties infected by PSTVd and to lay the foundation for the integrated control and breeding of potatoes for the disease resistance to PSTVd.