针刺俞募穴治疗功能性便秘的EGC及ENS-ICC-SMC功能网络调整机制研究

功能性便秘是消化系统的常见病、多发病，严重影响患者生活质量，药物治疗不理想，是目前亟待解决的临床热点问题。针灸治疗功能性便秘疗效确切，证据充分，是WHO推荐针灸治疗的43种疾病之一，其中大肠俞募穴是最经典、最常用的取穴方法，然而俞募穴治疗该病的作用机制尚未阐释清楚。最新研究表明EGC减少进而引起ENS-ICC-SMC功能网络失调是功能性便秘发病的关键环节，从外周机制入手可能是阐释作用机制的突破口。故本项目以针灸俞募穴理论为指导，以功能性便秘模型小鼠为研究对象，从影响胃肠动力的关键环节EGC、ENS-ICC-SMC功能网络、以及密切相关的GDNF-PI3K-Akt信号转导通路入手，运用免疫组化、原位杂交结合计算机图像分析、荧光定量PCR等方法，并采用基因敲除技术，从正、反两面证实针刺俞募穴对模型小鼠肠道动力、肠道功能网络的调整作用，拟深入阐释俞募穴治疗胃肠疾病这一针灸经典用穴规律的科学内涵。

Functional constipation (FC) is a common ailment and frequently encountered disease in department of gastroenterology, which is seriously reducing patients' quality of life. However, effect of medication treatment is not satisfactory and a better solution is badly needed. Acupuncture has been sufficiently proved effective in treating FC by clinical practice. FC is among 43 diseases recommended by WHO to be treated by acupuncture. Back-Shu and Front-Mu acupoints is a classical acupuncture approach for treating FC, but the mechanism behind has not been explained essentially. Recent studies indicate that the key point in pathogenesis of FC is decrease of EGC and unbalance of ENS-ICC-SMC functional network. Exploring the peripheral mechanism of acupuncture is probably a way to reach a breakthrough. Therefore, this project based on acupuncture Front-mu and Back-shu, a classic combination of acupuncture points, takes the functional constipated model mice as research subjects, further explains the scientific content of acupuncture in treating gastrointestinal disorders. The project starts with EGC, ENS-ICC-SMC network, and GDNF-PI3K-Akt signal transduction pathway, which are important factors effecting on gastrointestinal motility, then utilizes techniques such as immunohistochemistry, situ hybridization, quantitative image analysis performed with computer, fluorescence quota PCR and gene knockout to prove the regulation effect of acupuncture on gastrointestinal motility and functional network of FC model mice pros and cons.