甘蔗是以收获茎秆为主的最重要的糖料作物，分蘖是增加有效茎数的最有效途径，适当调控甘蔗分蘖成茎的比例是决定产量的关键。前期研究发现外源PP333浸种处理能显著促进甘蔗分蘖，而外源激素GA3 对甘蔗分蘖具有明显抑制作用；同时分析了GA3和PP333浸种处理后相关内源激素的变化规律。在此基础上，本申请项目拟通过RNA-Seq分析GA3、PP333和清水处理的甘蔗在分蘖期的差异，分析归类所获得的差异表达基因，并对高可信的靶基因进行GO功能注释和Pathway注释；发掘影响甘蔗分蘖的关键基因，鉴定关键基因的功能，构建影响甘蔗分蘖的分子调控网络，从分子水平上阐明植物生长调节剂调控甘蔗分蘖形成的分子调控机制，为甘蔗分子辅助育种及高产栽培提供理论依据。

Sugarcane is the most important sugar crop which stalks are harvested. Promoting sugarcane tillering is one of the most effective ways to increase the number of millable canes. It is very important to regulate sugarcane tillering developing millable canes for its yield formation. Our previous studies had found that tillering was significantly promoted when sugarcane buds were treated with plant growth regulators PP333, comparing with GA3, and then we analyzed the dynamic and correlation of endogenous phytohormones contents in sugarcane treated with GA3 and PP333. But practically their molecular regulatory mechanisms still remain unclear. In this study, we will employ RNA-Seq to analyze the transcriptomic dynamic changes of GA3 and PP333 treating sugarcane buds at different tillering stages. Meanwhile the differentially expressed genes are to be analyzed and categorized by GO functional annotations and Pathway annotations methods. Then we could find out the key genes and drawn their gene regulation networks, and will identify the biological functions of these key genes to elucidate the molecular mechanisms of regulation tillering in sugarcane treated with plant growth regulator. Our jobs will parse molecular regulation mechanism of sugarcane tillering, also provide a reference to molecular assisted breeding and chemical control technology for high yield cultivation in sugarcane.