基于Clarity技术的鼠脑基底节区三维化学构筑的计算机仿真模型构建

基底节区在运动调节中发挥重要作用，该区不同程度的损伤或功能紊乱，是许多运动功能障碍疾病的病理生理基础。帕金森病是一种以运动障碍为主要临床特征的神经系统退行性疾病，随着社会人口的老龄化，帕金森病日趋成为威胁人类健康、严重影响中老年人生活的常见疾病。深部脑电刺激技术（DBS）被认为是帕金森治疗的最后手段，但其机制尚不清楚。而基底节是DBS的主要靶点区。本课题拟用calrity技术，把在运动中起重要调节作用的基底神经节环路作为研究对象，通过免疫荧光、组化及激光共聚焦等方法在小鼠脑基底节区显示多巴胺能神经元和胆碱能神经元、受体及纤维连接，在计算机上建立基于化学构筑的基底神经节区三维计算机模型，分析神经元细胞密度及各神经元间密度关系，在计算机上模拟帕金森病，以探讨其发病机制？同时也为研究DBS机制建立新的技术平台，为DBS提供精确靶点。

The basal ganglia plays an important role in the adjustment of the movement. The different damage degrees of it is the pathological physiological basis of many motor dysfunction disease. Parkinson's disease is a kind of movement disorders as the main clinical characteristics and is the nervous system degenerative disease of lacking dopaminergic neurons, along with the social aging, Parkinson's disease has become increasingly and been a threat to human health, which is the serious influence in the life of the common diseases in the elderly. The structures of the basal nucleus are numerous and the relationship of them are complex. In order to realize virtual simulation of the basal nucleus on the computer, the key is the construction of the quantitative three-dimensional chemoarchitecure model of the area of.Our study will use clarity technology , and immunohistochemical, immune fluorescent, confocal laser scanning microscopy and electron microscopy in mouse to display the main neurons of basal nucleus, and With digital technology, constructing the three-dimensional chemoarchitecure model of basal nucleus to analyse neuron density and the density relation between neurons,providing a new way and technical platform for simulation of deep brain stimulation on the computer, to exploring the treatment mechanism.