侵袭转移是食管鳞癌（ESCC）发展的重要因素，Wnt/β-catenin信号通路的异常激活是肿瘤血管生成、侵袭转移的关键环节。我们前期研究中发现Dvl3可通过Wnt/β-catenin信号通路抑制食管鳞癌的侵袭转移，VEGF和Dvl3的表达在ESCC中存在相关性，因此我们推测，抑制VEGF和Dvl3在治疗ESCC中可能存在协同作用。针对ESCC的核心病机“痰瘀互结”，课题组研发了以化痰祛瘀为治则的院内制剂中药复方豆根管食通，临床研究发现该药对ESCC有确切疗效，同时基础实验证实其可通过抑制ESCC细胞的增殖、促进凋亡发挥抗肿瘤作用，并可同时下调VEGF和DVL3的表达。本课题运用构建慢病毒载体和siRNA干扰等技术，运用豆根管食通从体内、外两方面验证在ESCC中通过Wnt/β-catenin信号通路协同抑制VEGF与Dvl3进而发挥抗肿瘤作用的科学假说。

Invasion and metastasis are the key factors in the development of Esophageal squamous carcinoma (ESCC), abnormal activation of Wnt/β-catenin signaling pathway is the key of tumor angiogenesis, invasion and metastasis. In our preliminary experiment, Dvl3 could suppress invasion and metastasis in ESCC through Wnt/β-catenin signaling pathway, and the correlation between expression of VEGF and Dvl3 was also found in ESCC. Therefore, we consider that the synergistic effect may exist in the treatment of ESCC about suppression of expression of Dvl3 and VEGF. Turbid phlegm and blood stasis is a principal pathogenesis of ESCC, our group found one traditional Chinese Herb, Dougen Guanshitong Oral Liquid, which have curative effect on removing Phlegm and Blood Stasis (HTQY). Clinical research found that the drug has obvious curative effect on ESCC, and the basic experiment confirmed that it could inhibit tumor through restraining the proliferation , promoting apoptosis and reducing the expression of VEGF and Dvl3 in ESCC. The technology of slow virus vector. construction and siRNA jamming were used in this research. The scientific hypothesis proposed by us is that VEGF and Dvl3 have the coordinate repression on ESCC based on Wnt/β-catenin pathway. It was verified through vitro and vivo experiment by Dougen Guanshitong Oral Liquid.