天然活性羊膜微型支架动态调控慢性创面免疫炎症、重塑组织修复再生的实验研究

适宜的免疫炎症反应是损伤修复和组织重塑的基础，慢性创面的本质是正常有序的生物学愈合过程出现了紊乱，进入了一种动态变化的病理性炎症反应失调状态,因此目前通过调控单一炎症因子或信号通路的治疗效果有限。前期研究发现羊膜上皮细胞不仅可通过旁分泌作用促进皮肤损伤修复，同时具备免疫耐受和免疫调节可塑性的独特优势。我们在前期研究中初步制备了具有三维立体结构、保留上皮细胞活性的羊膜微型支架，其不仅能够分泌释放更丰富的活性因子，而且能够更好维持干细胞特性，发挥其免疫调节的功能。本项目拟进一步采用微型组织工程技术，结合自主研发的自动化高精度微粒皮制备机，构建最佳效价比大小的活性羊膜微型支架，并建立活性微型支架的冻存技术方案，探索其动态调控慢性创面免疫炎症反应、重塑组织修复再生的作用及机制，这是在新结题国家自然基金项目和发表2篇SCI论文基础上的延续和深入，该研究有望为慢性创面治疗提供新的方法和途径。

Appropriate immune inflammation is the basis of wound repairing and tissue remodeling. The cause of chronic wound is the disorder of normal biological healing process, which leads to a dynamic imbalance state of pathological inflammatory reaction. Therefore, the effect of regulating a single inflammatory factor or signal pathway which is adopted at present is not perfect. Previous studies have found that amniotic epithelial cells not only promote the repairing of skin injury through paracrine effects, but also have the unique advantages of immune tolerance and immune plasticity. Based on it, we preliminary prepare amniotic membrane microparticles with three-dimensional structure and viable epithelial cells, which not only secrete more active factors, but also better maintain the characteristics of stem cells to regulate the immune inflammation. The project intends to further adopt micro-tissue engineering technology, combining with the self-developed automatic high-precision cutting machine, to construct the optimal cost-effective size of the living micro-amniotic dermal scaffolds, which may dynamic regulate immune inflammation and reconstruct wound repairing through its epithelial cells and natural extracellular matrix components. The present project is a further study on the basis of previous National Natural Science Foundation and two papers that have been published on journals of Science Citation Index(SCI). The result expected to provide a new way for the treatment of chronic wounds.