针对电化学厌氧消化（EAD）中微生物电解池（MEC）的作用机制认识不足的问题，本项目首先在EAD混合培养体系中引入代谢通量分析，利用代谢通量分析软件（CellNetAnalyzer）辅助，构建EAD代谢网络及计量矩阵分析模型，并通过实验加以验证和调整；其次采用代谢通量分析、微生物分析和酶活分析三者相结合的方法，研究电化学参数扰动对EAD代谢网络中各代谢途径的代谢通量分布、相关微生物和相关酶活的影响，明确各代谢途径中物质流向及流量的变化特点、关键代谢节点处微生物的变化特征和相关酶活的变化规律，以及三者之间的联系；最后分别从化学计量学、微生物学和酶学的角度，阐明EAD的代谢途径和MEC的作用机制，从而对MEC的引入之所以能够有效地促进厌氧消化提高甲烷转化率给出一个合理的阐释，为EAD生产高品质生物天然气的代谢工程设计提供理论依据和调控方法。

Considering the lack-understanding of mechanisms of microbial electrolysis cell (MEC) in electrochemically anaerobic digestion (EAD), this project will firstly introduce metabolic flux analysis (MFA) into a mixed culture system to construct the metabolic network and metrological matrix analysis model of EAD by using the metabolic flux analysis software (CellNet Analyzer), and then the metabolic network and metrological matrix analysis model of EAD were verified and adjusted by experiments. Secondly, metabolic flux analysis, microbial analysis and enzyme activity analysis was used to investigate the effect of electrochemical parameters on the distribution of metabolic flux, related microorganisms and the related enzyme activities in EAD metabolic network, to clarify the changing characteristics of material flow direction and flow rate in various metabolic pathways, the changing features of microorganisms and the changing laws of enzyme activity at key metabolic nodes, as well as the relationship of them. Finally, the metabolic pathway of EAD and the mechanism of MEC will be illuminated from the view of chemometrics, microbiology and enzymology, respectively, to explain the MEC function of promoting anaerobic digestion and increasing methane conversion. It also provides a theoretical basis and regulation method for producing high quality biogas through metabolic engineering design of EAD.