搭载TNF-a的壳聚糖缓释系统的阻滞效应治疗先天性半椎体的研究

10岁以下出现的脊柱侧弯称为早发性脊柱侧弯（EOS），其中最常见的类型是先天性半椎体。因为此类病人年龄小且儿童具有生长发育的特点，目前治疗EOS的难度极大。但因脊柱侧弯导致的畸形不仅影响患者生存质量，甚至危及生命且对患者家属及社会造成严重负担，急需安全有效的治疗手段。本研究基于课题组在儿童脊柱畸形诊疗方面积累的丰富经验，结合前期国家自然科学基金资助EOS动物模型及手术治疗课题的研究基础；为探索生物学手段调节脊柱生长潜能而治疗EOS的技术，利用TNF-α对软骨细胞的诱导凋亡功能，以温度敏感型壳聚糖凝胶为载体，通过体外筛选适宜TNF-α浓度，及幼猪体内荧光检测扩散特征，构建合适配比及浓度的可注射缓释系统；对生长板切除的半椎体侧凸猪模型进行微创注射干预，以明确该系统对脊柱生长和侧凸控制的影响，为进一步探索促进/抑制双向调节生长的EOS生物治疗技术奠定基础。

Scoliosis that occurs under 10 years of age is called early onset scoliosis (EOS), the most common of which is the congenital hemivertebra. Because of the age of these patients and the growth and development of children, the current treatment of EOS is extremely difficult. However, the deformity caused by scoliosis not only affects the patient's quality of life, but is even life-threatening and imposes a serious burden on the patient's family and society. There is an urgent need for safe and effective treatment. This study is based on the extensive experience of the research team in the diagnosis and treatment of children with spinal deformity, combined with the research foundation of the previous National Natural Science Foundation funded EOS animal model and surgical treatment topics; the technology for the treatment of EOS by exploring biological means to regulate the growth potential of the spine. TNF-α induces apoptosis in chondrocytes, using temperature-sensitive chitosan gel as a carrier, screening for appropriate TNF-α concentration in vitro, and fluorescence detection and diffusion characteristics in young pigs to construct suitable ratios and concentrations. Injecting a slow-release system; minimally invasive injection of a semi-vertebral scoliosis pig model with a growth plate removed to determine the effect of the system on spinal growth and scoliosis control, to further explore the promotion/inhibition of bidirectional regulation of EOS biotherapy Technology lays the foundation.