由链格孢菌Alternaria alternata引起的枣黑斑病是南疆近几年暴发流行的一种新病害，给枣产业带来了重大损失。目前关于枣黑斑病菌的侵染致病机理尚无报道，为制定该病害有效防控对策带来了难度。本项目以南疆枣黑斑病菌（Alternaria alternata）为研究对象，采用根癌农杆菌介导遗传转化技术、连续切片和细胞染色技术研究Alternaria alternata在枣果组织细胞内的侵染过程及侵染后引起的病组织细胞的变化，明确该病菌侵染的形态变化机制；并对Alternaria alternata在侵染过程中产生的细胞壁降解酶和毒素进行分析，探讨Alternaria alternata侵染枣果的生理生化机制，以期为探究Alternaria alternata与寄主的互作及制定防控黑斑病策略提供理论基础。该项目的研究成果对保障南疆红枣产业的可持续发展具有重要的理论和实践意义.

Jujube blackspot infected by Alternaria alternata is a new disease that outbreaks widespreadly in southern Xinjiang in recent years. The pathogen infects late fruits and seriously threats to the development of jujube industry in southern Xinjiang. The infection law is theoretical basis of the disease prevention. However, there was no report about the research on infection process and mechanism of jujube blackspot at home and abroad and brough the difficulty to make preventive countermeasures. In this study, the infection expansion of jujube blackspot pathogen, Alternaria alternata, in fruit tissue and the change of disease tissue infected by the pathogen will be observed by using the agrobacterium-mediated transformation technique (ATMT), serial sectioning technique and staining technique. Furthermore, the cell wall-degrading enzymes and the toxin produced by Alternaria alternata will be analysised to investigate the physiological and biochemical mechanism of Alternaria alternata in order to provide a theoretical basis to explore the molecular mechanism and establish the strategy of prevention and control black spot further more. It has significance to the sustainable development of jujube industry in southern Xinjiang both theoretically and practically.