三维形貌配准结构约束的小动物模型DOT/FMT联合成像方法

荧光分子层析成像（FMT）结合了特异性荧光分子标记技术及近红外光谱学成像技术的优势，能够在体检测活体组织内部与细胞或者分子相关的生化反应过程，有望实现肿瘤的超早期诊断。由于受到系统性能的限制、有效背景光学参数缺失以及逆向问题高度不适定性的影响，现有FMT技术的空间分辨率、定量性以及鲁棒性尚待提高。本项目面向小动物全身成像应用，基于硅光电倍增管发展高灵敏度、高空间采样密度的形貌/扩散光学层析（DOT）/FMT联合测量系统，结合数字鼠形貌配准及形状DOT技术发展有效的解剖/光学结构获取方法，并在实现精准光子输运建模的基础上发展FMT先进图像重建方法。项目的实施有助于突破现有FMT方法的局限，提升FMT的空间分辨率及定量性，以期实现面向小动物全身成像的多模态联合成像先进技术。

Fluorescence molecular tomography, which combines the advantages of specific fluorescent molecular labeling technology and near-infrared spectroscopy imaging technology, can detect the biochemical reaction process related to cells or molecules in living tissues, and is expected to achieve ultra-early diagnosis of tumors. However, the spatial resolution, quantitativeness and robustness of the current FMT method are still need to be improved due to the limitation of system performance, the difficulty in obtaining effective background optical parameters and the high ill-posedness of the reconstruction process. Aimed at the application of the small animal whole-body imaging, the project intends to a develop a high sensitivity and high spatial sampling density multi-modality measurement system of morphology /diffusion optical tomography (DOT)/ FMT based on silicon photomultipliers, develop the effective methods for obtaining anatomical/optical structure with the combination of morphology registration and shape-parameterized DOT, and develop the advanced FMT image reconstruction methods based on the accurate photon transport model. The implementation of this project helps to break through the limitations of the current FMT methods and improve the spatial resolution and quantification of FMT, which is expected to realize the advanced multi-modal imaging technology for small animal whole-body imaging.