X染色体失活是维持物种内部遗传物质计量平衡的核心生物学过程。失活的X染色体（Xi）最终形成巴氏小体（无膜细胞器），Xi未被稳定失活会造成严重的病理缺陷，但是我们对其内在分子动力学特征的理解纬度尚不全面。根据初步的实验结果，我们试图通过HNRNPK蛋白为核心的液液相分离骨架，结合分子动力学模拟，经典遗传生物学手段以及生物信息学分析对X染色体失活延展进行深度挖掘。探究X染色体失活延展过程中结构蛋白元件，酶促催化元件，非编码RNA元件与染色质之间的立体构效关联，阐明X染色体失活过程的核心规律。

X chromosome inactivation is the core biological process that maintains the balance of the genetic material of a species. The inactivated X chromosome (Xi) eventually forms barr body (membrane-less organelle), the unstabilized inactivation of Xi chromosome will cause serious pathological defects, but our understanding of its intrinsic molecular dynamics is not comprehensive. According to the preliminary experimental results, we try to use the HNRNPK liquid liquid phase separation as scaffold, combine with molecular dynamics simulation, classical genetic biology methods and bioinformatics analysis to conduct in-depth exploration of X chromosome inactivation and spreading. Explore the three-dimensional structure-effect relationship between structural protein elements, enzymatic catalytic elements, non-coding RNA elements and chromatin during the inactivation and extension of X chromosome, and clarify the core laws of the X chromosome inactivation process.